Microscopy Group
We have established an imaging center at FDU with the purchase of a fluorescence microscopy system. This system was funded by a major research instrumentation grant (MRI) from the National Science Foundation.
Principal Investigator:
Patricia Melloy, Ph.D., Department of Biological and Allied Health Science; (Research interests: nuclear envelope and membrane dynamics in budding yeast)
Co-principal Investigators:
Michael Avaltroni, Ph.D., Department of Chemistry; (Development and testing of new materials for implantable medical devices)
Eric Iannacone, Ph.D., Department of Biological and Allied Health Sciences; (Neural development in Drosophila)
The Fluorescence Microscopy system:
Our system includes a Leica Microsystems DM5500 microscope, with a Hamamatsu ORCA ER cooled CCD camera. We have the capability of visualizing many fluorescent markers or probes including GFP, CFP, YFP, DAPI, and Rhodamine. In addition, our system is equipped for bright-field and DIC light microscopy.
Links:
- Microscope: http://www.leica-microsystems.com/
- CCD camera: http://www.hamamatsu.com/
- Introduction to Microscopy: http://micro.magnet.fsu.edu/primer/
- Cell Biological Applications of fluorescence microscopy: http://www.itg.uiuc.edu/publications/techreports/99-006/
- American Society for Cell Biology: www.ascb.org
- National Science Foundation: www.nsf.gov
Collaborations:
We are open to establishing new collaborations within FDU and with other scientists and educators in the area. Please contact Dr. Patricia Melloy for inquiries or questions.
(pmelloy@fdu.edu, 973-443-8757)
Sample Images:
A.
B.
C.
D.
E.
F.
Sample images taken using the fluorescence microscopy system.
Shown in A-C are bright-field images of Drosophila embryos stained with antibodies to highlight nerve cell bodies and the ventral nerve cord (20X objective). Images in D-F are yeast cells observed using DIC (A) and fluorescence microscopy to observe the GFP signal (E) and the Hoechst DNA stain (F) (100X objective).
